Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 17516-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis using 17516-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 17516-1-AP
IP result of anti-Cytokeratin 17-Specific (IP:17516-1-AP, 4ug; Detection:17516-1-AP 1:1000) with A431 cells lysate 1200ug.
IHC staining of human cervical squamous cancer using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical squamous cancer using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung squamous cell carcinoma using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung squamous cell carcinoma using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17516-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17516-1-AP (Cytokeratin 17-Specific antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17516-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 17516-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 17-Specific antibody (17516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Cytokeratin 17-Specific Polyclonal antibody

Cytokeratin 17-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17516-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Cytokeratin 17, KRT17, CK 17, CK-17, Cytokeratin,Keratin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (29)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, HaCaT cells, mouse skin tissue, rat skin tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human cervical squamous cancer tissue, human bowen disease tissue, human lung squamous cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:20000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 16 publications below
IHC	See 12 publications below
IF	See 7 publications below
CoIP	See 1 publications below

Product Information

17516-1-AP targets Cytokeratin 17-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	keratin 17
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa
GenBank accession number	NM_000422
Gene Symbol	Cytokeratin 17
Gene ID (NCBI)	3872
RRID	AB_2133036
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q04695
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 17-Specific antibody 17516-1-AP	Download protocol
IHC protocol for Cytokeratin 17-Specific antibody 17516-1-AP	Download protocol
IP protocol for Cytokeratin 17-Specific antibody 17516-1-AP	Download protocol
WB protocol for Cytokeratin 17-Specific antibody 17516-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		Science Spatiotemporal immune zonation of the human kidney. Authors - Benjamin J Stewart View Article
human	IHC	Clin Cancer Res A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer. Authors - Yifan Wang View Article
human	WB	Breast Cancer Res Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation. Authors - Dongdong Wang View Article
human	WB	Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer Authors - Mengjie Li KD Validated View Article
mouse	WB	Phytother Res Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization Authors - Yongling Ning View Article
mouse,human	WB	J Invest Dermatol Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage Authors - ZhiCao Yue View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17516-1-AP

WB analysis using 17516-1-AP

IP experiment of A431 using 17516-1-AP

IHC staining of human cervical squamous cancer using 17516-1-AP

IHC staining of human cervical squamous cancer using 17516-1-AP

IHC staining of human lung squamous cell carcinoma using 17516-1-AP

IHC staining of human lung squamous cell carcinoma using 17516-1-AP

IHC staining of human bowen disease using 17516-1-AP

IHC staining of human bowen disease using 17516-1-AP

IF Staining of HepG2 using 17516-1-AP

IF Staining of HeLa using 17516-1-AP

Cytokeratin 17-Specific Polyclonal antibody

Cytokeratin 17-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spatiotemporal immune zonation of the human kidney.

A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.

Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization

Keratin 17 Impacts Global Gene Expression and Controls G2/M Cell Cycle Transition in Ionizing Radiation-Induced Skin Damage