Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A431 using 10137-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10137-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10137-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10137-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10137-1-AP
IP result of anti-Cytokeratin 15 (IP:10137-1-AP, 4ug; Detection:10137-1-AP 1:8000) with A431 cells lysate 1000 ug.
IHC staining of human cervical cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10137-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10137-1-AP (Cytokeratin 15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10137-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (10137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10137-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KRT15, keratin 15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 7 publications below
IF	See 10 publications below

Product Information

10137-1-AP targets Cytokeratin 15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0185 Product name: Recombinant human KRT15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-450 aa of BC002641 Sequence: AYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVS 相同性解析による交差性が予測される生物種
Full Name	keratin 15
Calculated molecular weight	49 kDa
Observed molecular weight	49 kDa
GenBank accession number	BC002641
Gene Symbol	KRT15
Gene ID (NCBI)	3866
RRID	AB_2234359
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19012
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
IF protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
IHC protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
IP protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol
WB protocol for Cytokeratin 15 antibody 10137-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Biomater Sci A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring Authors - Ximeng Kang View Article
mouse	IF	Stem Cells LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis. Authors - R Sartaj View Article
human	IF	J Invest Dermatol GW bodies: cytoplasmic compartments in normal human skin. Authors - Zee Jennifer M JM View Article
human	WB	Carcinogenesis Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein. Authors - Gautam Adhikary View Article
mouse	IF	J Ethnopharmacol Dahuang-Gancao Decoction Ameliorates Testosterone-Induced Androgenetic Alopecia in Mice Authors - Huanzhu Qian View Article
mouse	IF	Sci Rep Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers. Authors - R Sartaj View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 10137-1-AP

WB analysis of HeLa using 10137-1-AP

WB analysis of PC-3 using 10137-1-AP

WB analysis of mouse thymus using 10137-1-AP

IP experiment of A431 using 10137-1-AP

IHC staining of human cervical cancer using 10137-1-AP

IHC staining of human cervical cancer using 10137-1-AP

IHC staining of human oesophagus cancer using 10137-1-AP

IHC staining of human oesophagus cancer using 10137-1-AP

IHC staining of human breast cancer using 10137-1-AP

IHC staining of human breast cancer using 10137-1-AP

IF Staining of HeLa using 10137-1-AP

Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring

LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.

GW bodies: cytoplasmic compartments in normal human skin.

Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.

Dahuang-Gancao Decoction Ameliorates Testosterone-Induced Androgenetic Alopecia in Mice

Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.