KRT12 Polyclonal antibody
KRT12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24789-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KRT12 Ready-To-Use IHC Kit. KRT12 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 6 publications below |
Product Information
24789-1-AP targets KRT12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KRT12 fusion protein Ag20312 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 12 |
| Calculated molecular weight | 494 aa, 54 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC156641 |
| Gene symbol | KRT12 |
| Gene ID (NCBI) | 3859 |
| RRID | AB_2879726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KRT12 antibody 24789-1-AP | Download protocol |
| IHC protocol for KRT12 antibody 24789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. | ||
Exp Eye Res Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye. | ||
Int Ophthalmol ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway | ||
J Ocul Pharmacol Ther ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway | ||
Transl Vis Sci Technol Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500 |
