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  • Featured Product
  • KD/KO Validated

KPNA3 Monoclonal antibody

KPNA3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3D2

Cat no : 67892-1-Ig

Synonyms

hSRP1, Importin alpha Q2, Importin subunit alpha 3, IPOA4, Karyopherin subunit alpha 3, KPNA3, Qip2, SRP1, SRP1 gamma, SRP1gamma, SRP4

Validation Data Gallery

Applications:
  • WB analysis using 67892-1-Ig

    WB analysis using 67892-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67892-1-Ig

    WB analysis of HepG2 using 67892-1-Ig

    WB result of KPNA3 antibody (67892-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA3 transfected HepG2 cells.

  • WB analysis using 67892-1-Ig

    WB analysis using 67892-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67892-1-Ig (KPNA3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse testis using 67892-1-Ig

    IHC staining of mouse testis using 67892-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included
  • IHC staining of mouse testis using 67892-1-Ig

    IHC staining of mouse testis using 67892-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67892-1-Ig (KPNA3 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA3 antibody included

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KPNA3 Ready-To-Use IHC Kit. KPNA3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Sample-dependent, check data in validation data gallery

Product Information

67892-1-Ig targets KPNA3 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KPNA3 fusion protein Ag27767 相同性解析による交差性が予測される生物種
Full Name karyopherin alpha 3 (importin alpha 4)
Calculated molecular weight 58 kDa
Observed molecular weight58 kDa
GenBank accession numberBC017355
Gene symbol KPNA3
Gene ID (NCBI) 3839
RRIDAB_2918648
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

karyopherin subunit alpha 3 (KPNA3) is a member of the importin alpha family which can transport molecules between the mucleus and cytoplasm. It is ubiquitous expressed in brain,testis and esophagus. KPNA3 is associated with many severe diseases such as hepatocellular Carcinoma (PMID: 31417635) , schizophrenia, major depression and opiate dependence (PMID: 22960338) . The molecular weight of KPNA3 is about 58 kDa.

Protocols

Product Specific Protocols
WB protocol for KPNA3 antibody 67892-1-IgDownload protocol
IHC protocol for KPNA3 antibody 67892-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Daulet (Verified Customer) (02-09-2022)

It worked on the lysates of SH-SY5Y cells. So, if you are going to use it on this sample, then I can recommend it. However, bear in mind, that whether it will work or not depends on how you treat your samples and other protocol niceties.

  • Applications: Western Blot
  • Cell Tissue Type: SH-SY5Y