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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse KLRG1 (2F1)

KLRG1 Monoclonal Antibody for FC

Host / Isotype

Golden Syrian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F1

Cat no : CL647-65084

Synonyms

2F1 Ag, Klrg1, MAFA, MAFA L

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL647-65084

    FC experiment of mouse splenocytes using CL647-65084

    1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and 5 ul CoraLite® Plus 647 Anti-Mouse KLRG1 (CL647-65084, Clone: 2F1). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-65084

    FC experiment of mouse splenocytes using CL647-65084

    1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse NK1.1 (CD161) (FITC-65138, Clone: PK136) and CoraLite® Plus 647 conjugated Hamster IgG isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65084-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65084 targets KLRG1 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Golden Syrian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily G, member 1
GenBank accession numberBC101953
Gene symbol Klrg1
Gene ID (NCBI) 50928
RRIDAB_2920251
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 KLRG1 antibody CL647-65084Download protocol
Standard Protocols
Click here to view our Standard Protocols