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  • Featured Product
  • KD/KO Validated

KLHL18 Polyclonal antibody

KLHL18 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17229-1-AP

Synonyms

kelch like 18 (Drosophila), Kelch like protein 18, KIAA0795, KLHL18

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 17229-1-AP

    WB analysis of HepG2 using 17229-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17229-1-AP

    WB analysis of HeLa using 17229-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 17229-1-AP

    WB analysis of COLO 320 using 17229-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 17229-1-AP

    WB analysis of HL-60 using 17229-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 17229-1-AP

    WB analysis of mouse brain using 17229-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 17229-1-AP

    WB analysis of HEK-293 using 17229-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 17229-1-AP

    IHC staining of human brain using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17229-1-AP

    IHC staining of human brain using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 17229-1-AP

    IF Staining of HeLa using 17229-1-AP

    Immunofluorescent analysis of Hela cells, using KLHL18 antibody 17229-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17229-1-AP is KLHL18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL18 fusion protein Ag11070 相同性解析による交差性が予測される生物種
Full Name kelch-like 18 (Drosophila)
Calculated molecular weight 509 aa, 56 kDa
Observed molecular weight 63 kDa, 56 kDa
GenBank accession numberBC032620
Gene symbol KLHL18
Gene ID (NCBI) 23276
RRIDAB_10665554
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL18 antibody 17229-1-APDownload protocol
IHC protocol for KLHL18 antibody 17229-1-APDownload protocol
IF protocol for KLHL18 antibody 17229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Biosci

KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity.

Authors - Xizi Jiang
View Article
humanWB

Biol Open

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Authors - Moghe Saili S
  • KD Validated
View Article