Validation Data Gallery

Filter: AllWBIHC

WB analysis of HepG2 using 17229-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17229-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17229-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17229-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 17229-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 17229-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 17229-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon using 17229-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17229-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17229-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

KLHL18 Polyclonal antibody

KLHL18 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17229-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

OK/SW-cl.74, Kelch-like protein 18

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse brain tissue
Positive IHC detected in	human colon tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

17229-1-AP targets KLHL18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLHL18 fusion protein Ag11070 相同性解析による交差性が予測される生物種
Full Name	kelch-like 18 (Drosophila)
Calculated molecular weight	509 aa, 56 kDa
Observed molecular weight	63 kDa, 56 kDa
GenBank accession number	BC032620
Gene Symbol	KLHL18
Gene ID (NCBI)	23276
RRID	AB_10665554
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94889
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL18 antibody 17229-1-AP	Download protocol
IHC protocol for KLHL18 antibody 17229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cell Biosci KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity. Authors - Xizi Jiang View Article
human	WB	Biol Open The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A. Authors - Moghe Saili S KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 17229-1-AP

WB analysis of mouse brain using 17229-1-AP

WB analysis of HEK-293 using 17229-1-AP

WB analysis of HeLa using 17229-1-AP

WB analysis of COLO 320 using 17229-1-AP

WB analysis of HL-60 using 17229-1-AP

IHC staining of human colon using 17229-1-AP