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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated KIF5A Monoclonal antibody

KIF5A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7E3

Cat no : CL488-67009

Synonyms

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

Validation Data Gallery

Applications:
  • IF Staining of PC-12 using CL488-67009

    IF Staining of PC-12 using CL488-67009

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite® Plus 488 KIF5A antibody (CL488-67009, Clone: 1A7E3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 67009-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67009 targets KIF5A in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIF5A fusion protein Ag15682 相同性解析による交差性が予測される生物種
Full Name kinesin family member 5A
Calculated molecular weight 1032 aa, 117 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC146670
Gene symbol KIF5A
Gene ID (NCBI) 3798
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KIF5A antibody CL488-67009Download protocol
Standard Protocols
Click here to view our Standard Protocols