Validation Data Gallery
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Tested Applications
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
12139-1-AP targets KIF2C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2786 Product name: Recombinant human KIF2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-518 aa of BC014924 Sequence: EEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSGQTFANSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINK 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 2C |
| Calculated molecular weight | 725 aa, 81 kDa |
| Observed molecular weight | 81 kDa |
| GenBank accession number | BC014924 |
| Gene Symbol | KIF2C |
| Gene ID (NCBI) | 11004 |
| RRID | AB_2877829 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99661 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF2C antibody 12139-1-AP | Download protocol |
| IP protocol for KIF2C antibody 12139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization. | ||
Cancer Commun (Lond) CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer. | ||
J Physiol Ablation of KIF2C in Purkinje cells impairs metabotropic glutamate receptor trafficking and motor coordination in male mice | ||
Front Cell Dev Biol Integrative Analysis of Biomarkers Through Machine Learning Identifies Stemness Features in Colorectal Cancer. | ||
Front Oncol Novel prognostic biomarkers in nasopharyngeal carcinoma unveiled by mega-data bioinformatics analysis | ||
Eur J Med Res Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells |
