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  • KD/KO Validated

KIF20A Polyclonal antibody

KIF20A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15911-1-AP

Synonyms

GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6



Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15911-1-AP targets KIF20A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF20A fusion protein Ag8700 相同性解析による交差性が予測される生物種
Full Name kinesin family member 20A
Calculated molecular weight 890 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC012999
Gene symbol KIF20A
Gene ID (NCBI) 10112
RRIDAB_2131434
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Protocols

Product Specific Protocols
WB protocol for KIF20A antibody 15911-1-APDownload protocol
IHC protocol for KIF20A antibody 15911-1-APDownload protocol
IP protocol for KIF20A antibody 15911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.

Authors - Stéphanie Miserey-Lenkei
humanWB

Proteomics

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.

Authors - Yan Guang-Rong GR
humanIHC

J Cancer

Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.

Authors - Lingyu Zhang

J Biochem Mol Toxicol

Bisphenol A triggers the malignancy of acute myeloid leukemia cells via regulation of IL-4 and IL-6.

Authors - Suwei Zhang
humanWB

Dis Markers

KIF20A Predicts Poor Survival of Patients and Promotes Colorectal Cancer Tumor Progression through the JAK/STAT3 Signaling Pathway.

Authors - Qi Zhang
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Juliane (Verified Customer) (10-25-2022)

Golgi and nucleus staining ok but cytoplasmic difficult

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
KIF20A Antibody Immunofluorescence validation (1:500 dilution) in HeLa (Cat no:15911-1-AP)