Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 12 publications below |
| WB | See 31 publications below |
| IHC | See 14 publications below |
| IF | See 8 publications below |
Product Information
55407-1-AP targets KIF15 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 15 |
| Calculated molecular weight | 160 kDa |
| Observed molecular weight | 150-165 kDa |
| GenBank accession number | NM_020242 |
| Gene Symbol | KIF15 |
| Gene ID (NCBI) | 56992 |
| RRID | AB_11182836 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NS87 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KIF15 antibody 55407-1-AP | Download protocol |
| IHC protocol for KIF15 antibody 55407-1-AP | Download protocol |
| IP protocol for KIF15 antibody 55407-1-AP | Download protocol |
| WB protocol for KIF15 antibody 55407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.
| ||
Am J Respir Crit Care Med Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis | ||
Cell Death Dis B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer.
| ||
Int J Biol Sci KIF15 promotes human glioblastoma progression under the synergistic transactivation of REST and P300
| ||
Int J Biol Sci ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer. | ||
J Transl Med Inhibition of the FEN1-PBX1 axis elicits cellular senescence in breast cancer via the increased intracellular reactive oxygen species levels |