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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIF15 Polyclonal antibody

KIF15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30774-1-AP

Synonyms

FLJ25667, HKLP2, KIF15, kinesin family member 15, Kinesin like protein 2, Kinesin like protein 7, Kinesin like protein KIF15, KLP2, KNSL7, NY BR 62

Validation Data Gallery

Applications:
  • WB analysis using 30774-1-AP

    WB analysis using 30774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30774-1-AP (KIF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cels, K-562 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

30774-1-AP targets KIF15 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF15 fusion protein Ag33897 相同性解析による交差性が予測される生物種
Full Name kinesin family member 15
Calculated molecular weight160 kDa
Observed molecular weight145-150 kDa
GenBank accession numberNM_020242
Gene symbol KIF15
Gene ID (NCBI) 56992
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. It is located in the cytoplasm and biased expression in testis. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15 (14618103). It is known that KIF15 participates in important events during neuronal development and involves multiple cellular processes such as proliferation, apoptosis, and differentiation. There is emerging evidence indicating that KIF15 plays an important role in several malignancies including pancreatic cancer, hepatocellular carcinoma, and breast cancer (PMID: 33277366).

Protocols

Product Specific Protocols
WB protocol for KIF15 antibody 30774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols