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  • KD/KO Validated

UFL1 Polyclonal antibody

UFL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26087-1-AP

Synonyms

E3 UFM1 protein ligase 1, KIAA0776, RP3 393D12.1, UFL1



Tested Applications

Positive WB detected inHEK-293T cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UFL1 Ready-To-Use IHC Kit. UFL1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

26087-1-AP targets UFL1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UFL1 fusion protein Ag23404 相同性解析による交差性が予測される生物種
Full Name KIAA0776
Observed molecular weight80 kDa; 100 kDa
GenBank accession numberBC036379
Gene symbol KIAA0776
Gene ID (NCBI) 23376
RRIDAB_2880370
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UFL1 antibody 26087-1-APDownload protocol
IHC protocol for UFL1 antibody 26087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
mouseWB,IF

Oxid Med Cell Longev

UFM1-Specific Ligase 1 Ligating Enzyme 1 Mediates Milk Protein and Fat Synthesis-Related Gene Expression via the JNK Signaling Pathway in Mouse Mammary Epithelial Cells.

Authors - Meiqian Kuang
  • KO Validated
bovineWB,IHC,IF

Cell Stress Chaperones

Protective effects of UFL1 against endoplasmic reticulum stress-induced autophagy in bovine mammary epithelial cells.

Authors - Meiqian Kuang
  • KD Validated
bovineWB

In Vitro Cell Dev Biol Anim

UFL1 regulates milk protein and fat synthesis-related gene expression of bovine mammary epithelial cells probably via the mTOR signaling pathway.

Authors - Chengmin Li
  • KD Validated
bovineIF,IHC

Biomolecules

UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells.

Authors - Xinling Wang
bovineWB,IF,IHC

Oxid Med Cell Longev

UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells.

Authors - Chengmin Li
  • KD Validated