Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 12345-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 12345-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12345-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12345-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12345-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12345-1-AP
IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug.
IHC staining of mouse brain using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
IHC staining of human testis using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
IHC staining of human breast cancer using 12345-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
IF Staining of MCF-7 using 12345-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 12345-1-AP
Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12345-1-AP

Publications(61)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

DYNC1H 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (61)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 36 publications below
IHC	See 7 publications below
IF	See 18 publications below
IP	See 4 publications below
CoIP	See 1 publications below

Product Information

12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	human, mouse, rat, pig, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2999 Product name: Recombinant human KIAA0325 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 590-989 aa of BC021297 Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE 相同性解析による交差性が予測される生物種
Full Name	dynein, cytoplasmic 1, heavy chain 1
Calculated molecular weight	4646 aa, 532 kDa
Observed molecular weight	532 kDa
GenBank accession number	BC021297
Gene Symbol	DYNC1H1
Gene ID (NCBI)	1778
RRID	AB_2261765
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14204
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

Protocols

Product Specific Protocols
IF protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
IHC protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
IP protocol for DYNC1H1 antibody 12345-1-AP	Download protocol
WB protocol for DYNC1H1 antibody 12345-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Science Mechanism of spindle pole organization and instability in human oocytes. Authors - Chun So View Article
mouse	IF	Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Authors - Chun So View Article
human	WB	Nature Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Authors - Henri-François Renard KD Validated View Article
human	WB	Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. Authors - Chuchu Zhou View Article
human		Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5. Authors - Andrew Kodani View Article
human	WB	J Cell Biol Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. Authors - Hironori Inaba KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 12345-1-AP

WB analysis using 12345-1-AP

WB analysis of human brain using 12345-1-AP

WB analysis of human brain using 12345-1-AP

WB analysis of HeLa using 12345-1-AP

IP experiment of HeLa using 12345-1-AP

IHC staining of mouse brain using 12345-1-AP

IHC staining of mouse brain using 12345-1-AP

IHC staining of human colon using 12345-1-AP

IHC staining of human colon using 12345-1-AP

IHC staining of human testis using 12345-1-AP

IHC staining of human testis using 12345-1-AP

IHC staining of human breast cancer using 12345-1-AP

IF Staining of MCF-7 using 12345-1-AP

IF Staining of MCF-7 using 12345-1-AP

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mechanism of spindle pole organization and instability in human oocytes.

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.