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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1A3

Cat no : 67886-1-Ig

Synonyms

AOF1, bA204B7.3, C6orf193, dJ298J15.2, DKFZp686I0412, FLJ33898, FLJ34109, FLJ43328, KDM1B, LSD2

Validation Data Gallery

Applications:
  • WB analysis using 67886-1-Ig

    WB analysis using 67886-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 67886-1-Ig

    WB analysis of K-562 using 67886-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of C6 using 67886-1-Ig

    WB analysis of C6 using 67886-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67886-1-Ig

    WB analysis of NIH/3T3 using 67886-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of SW 1990 using 67886-1-Ig

    FC experiment of SW 1990 using 67886-1-Ig

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHCT 116 cells, K-562 cells, C6 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells
Positive FC detected inSW 1990 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67886-1-Ig targets KDM1B in WB, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KDM1B fusion protein Ag30672 相同性解析による交差性が予測される生物種
Full Name amine oxidase (flavin containing) domain 1
Calculated molecular weight 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberNM_001364614
Gene symbol AOF1
Gene ID (NCBI) 221656
RRIDAB_2918643
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KDM1B antibody 67886-1-IgDownload protocol
FC protocol for KDM1B antibody 67886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols