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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KANK1 Monoclonal antibody

KANK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D3G8

Cat no : 68613-1-Ig

Synonyms

ANKRD15, DKFZp451G231, KANK, KANK1, KIAA0172

Validation Data Gallery

Applications:
  • WB analysis using 68613-1-Ig

    WB analysis using 68613-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68613-1-Ig (KANK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA375 cells, PC-3 cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, HuH-7 cells, Daudi cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy KANK1 Ready-To-Use IHC Kit. KANK1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68613-1-Ig targets KANK1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KANK1 fusion protein Ag31440 相同性解析による交差性が予測される生物種
Full Name KN motif and ankyrin repeat domains 1
Calculated molecular weight147KD
Observed molecular weight130-200 kDa
GenBank accession numberNM_015158
Gene symbol KANK1
Gene ID (NCBI) 23189
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

Protocols

Product Specific Protocols
WB protocol for KANK1 antibody 68613-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols