Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 27189-1-AP
WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.
WB analysis using 27189-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 27189-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27189-1-AP
WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.
WB analysis of A431 using 27189-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skin using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 27189-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin alpha 6 antibody (27189-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

Integrin alpha 6 Polyclonal antibody

Integrin alpha 6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27189-1-AP

Publications(41)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ITGA6, CD49 antigen-like family member F, CD49f, Integrin alpha-6, Integrin alpha-6 heavy chain

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (41)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, A431 cells, HepG2 cells, mouse lung tissue, Calu-1 cells, SW480 cells, rat lung tissue
Positive IHC detected in	mouse skin tissue, human colon tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 22 publications below
IHC	See 11 publications below
IF	See 13 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

27189-1-AP targets Integrin alpha 6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25835 Product name: Recombinant human Integrin alpha-6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 477-690 aa of BC136456 Sequence: TPNRIDLRQKTACGAPSGICLQVKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLKRQKQKVCMEETLWLQDNIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSDEPKTAHIDVHFLKEGCGDDNVCNSNLKLEYKFCTREGNQDKFSYLPIQKGVPELVLKDQKDIALEITVTNSPSNPRNPTK 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 6
Calculated molecular weight	1073 aa, 119 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	BC136456
Gene Symbol	Integrin alpha 6
Gene ID (NCBI)	3655
RRID	AB_2880792
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P23229
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).

Protocols

Product Specific Protocols
IF protocol for Integrin alpha 6 antibody 27189-1-AP	Download protocol
IHC protocol for Integrin alpha 6 antibody 27189-1-AP	Download protocol
WB protocol for Integrin alpha 6 antibody 27189-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	ACS Nano Biomimetic Topological Micropattern Arrays Regulate the Heterogeneity of Cellular Fates in Lung Fibroblasts between Fibrosis and Invasion Authors - Xinglong Zhu View Article
human	WB	Trends Biotechnol A regenerative and programmable iPSC-derived platelet platform for universal drug delivery and immunotherapy. Authors - Lingna Wang View Article
		Theranostics Comprehensive exome profiling identifies ARHGEF12 mutation as a driver in gastric cancer with ovarian metastasis Authors - Mingda Zhang View Article
		Cell Rep Med DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription Authors - Tian Xia View Article
human	IHC	Cell Death Dis TM4SF1 promotes esophageal squamous cell carcinoma metastasis by interacting with integrin α6. Authors - Sicong Hou View Article
mouse	WB	Phytomedicine Proteomics reveals the pharmacological mechanism of flavonoids from Astragali Complanati Semen in preventing chronic liver injury Authors - Lin Chen View Article

Validation Data Gallery

WB analysis of A549 using 27189-1-AP

WB analysis using 27189-1-AP

WB analysis using 27189-1-AP

WB analysis of HepG2 using 27189-1-AP

WB analysis of A431 using 27189-1-AP

IHC staining of mouse skin using 27189-1-AP

IHC staining of mouse skin using 27189-1-AP

IHC staining of human colon using 27189-1-AP

IHC staining of human colon using 27189-1-AP

IHC staining of mouse colon using 27189-1-AP

IHC staining of mouse colon using 27189-1-AP

IF Staining of A431 using 27189-1-AP

Integrin alpha 6 Polyclonal antibody

Integrin alpha 6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Biomimetic Topological Micropattern Arrays Regulate the Heterogeneity of Cellular Fates in Lung Fibroblasts between Fibrosis and Invasion

A regenerative and programmable iPSC-derived platelet platform for universal drug delivery and immunotherapy.

Comprehensive exome profiling identifies ARHGEF12 mutation as a driver in gastric cancer with ovarian metastasis

DDX5 super-enhancer promotes vasculogenic mimicry formation and metastasis in nasopharyngeal carcinoma by enhancing ADAM10 transcription

TM4SF1 promotes esophageal squamous cell carcinoma metastasis by interacting with integrin α6.

Proteomics reveals the pharmacological mechanism of flavonoids from Astragali Complanati Semen in preventing chronic liver injury

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