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APC Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

MOPC-21

Cat no : APC-65124

Synonyms

APC Mouse IgG1 Isotype Control, IgG1, IgG1 Isotype Control

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using APC-65124

    FC experiment of human peripheral blood lymphocytes using APC-65124

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.50 ug APC Mouse IgG1 Isotype Control (APC-65124, clone: MOPC-21) (red) or with 0.50 ug APC Anti-Human CD4 (APC-65143, clone: RPA-T4) (orange), or not stained (blank) (blue). Cells were not fixed.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 2 publications below

Product Information

APC-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Gene symbol Ighg1
Gene ID (NCBI) 16017
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for APC IgG1 Isotype Control antibody APC-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Cell Signal

RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.

Authors - Wenjing Li
View Article
FC

Carbohydr Polym

Plasmablasts induced by chitosan oligosaccharide secrete natural IgM to enhance the humoral immunity in grass carp.

Authors - Jie Wang
View Article