Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 30000-0-AP

Synonyms

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin, RIGGCON



Tested Applications

Positive WB detected inProtein, Control
Positive IP detected inControl

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and N/A for WB
Sample-dependent, check data in validation data gallery

Product Information

30000-0-AP targets Rabbit IgG control in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N/A
Full Name Rabbit IgG control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

SpeciesApplicationTitle

Genome Biol

PMID: 30642385

Authors - Xing Qin

Biol Pharm Bull

VEGF antagonism attenuates cerebral ischemia/reperfusion-induced injury via inhibiting endoplasmic reticulum stress-mediated apoptosis.

Authors - Shu-Qing Feng

Med Sci Monit

Expression of TMEFF2 in Human Pancreatic Cancer Tissue and the Effects of TMEFF2 Knockdown on Cell, Proliferation, and Apoptosis in Human Pancreatic Cell Lines.

Authors - Kailiang Li

EBioMedicine

The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.

Authors - Siliang Wei

Free Radic Biol Med

Novel diterpenoid-type activators of the Keap1/Nrf2/ARE signaling pathway and their regulation of redox homeostasis.

Authors - Ai-Ling Li

J Exp Clin Cancer Res

USP7 is a novel Deubiquitinase sustaining PLK1 protein stability and regulating chromosome alignment in mitosis.

Authors - Yuchong Peng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anita (Verified Customer) (01-04-2022)

Works well as a control for IP

  • Applications: Immunoprecipitation
  • Cell Tissue Type: C. elegans
FH

Kenneth (Verified Customer) (02-14-2019)

Great company with Great products. Highly recommended.

  • Applications: Western Blot,
FH

Priyanka (Verified Customer) (12-18-2018)

  • Applications: Western Blot, Immunofluorescence,
FH

Seongjin (Verified Customer) (12-14-2018)

  • Applications: Immunoprecipitation,