CoraLite® Plus 488-conjugated Iduronate 2 sulfatase Monoclonal antibody
Iduronate 2 sulfatase Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat , pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A3F9
Cat no : CL488-66112
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66112 targets Iduronate 2 sulfatase in IF applications and shows reactivity with human, mouse, rat , pig samples.
| Tested Reactivity | human, mouse, rat , pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Iduronate 2 sulfatase fusion protein Ag19095 相同性解析による交差性が予測される生物種 |
| Full Name | iduronate 2-sulfatase |
| Calculated molecular weight | 312aa,35 kDa; 550aa,62 kDa |
| Observed molecular weight | 76 kDa, 55 kDa |
| GenBank accession number | BC006170 |
| Gene symbol | IDS |
| Gene ID (NCBI) | 3423 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Iduronate 2 sulfatase antibody CL488-66112 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
