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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IWS1 Polyclonal antibody

IWS1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16943-1-AP

Synonyms

IWS1, IWS1 homolog (S. cerevisiae), IWS1 like protein, IWS1L, Protein IWS1 homolog

Validation Data Gallery

Applications:
  • WB analysis using 16943-1-AP

    WB analysis using 16943-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16943-1-AP

    WB analysis of HeLa using 16943-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 16943-1-AP

    WB analysis of mouse brain using 16943-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 16943-1-AP

    WB analysis of rat brain using 16943-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 16943-1-AP

    WB analysis of human brain using 16943-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 16943-1-AP

    IP experiment of mouse brain using 16943-1-AP

    IP result of anti-IWS1 (IP:16943-1-AP, 4ug; Detection:16943-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
Positive IP detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy IWS1 Ready-To-Use IHC Kit. IWS1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

ChIPSee 1 publications below

Product Information

16943-1-AP targets IWS1 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IWS1 fusion protein Ag10554 相同性解析による交差性が予測される生物種
Full Name IWS1 homolog (S. cerevisiae)
Calculated molecular weight 819 aa, 92 kDa
Observed molecular weight 116-120 kDa
GenBank accession numberBC017012
Gene symbol IWS1
Gene ID (NCBI) 55677
RRIDAB_2128229
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IWS1 antibody 16943-1-APDownload protocol
IP protocol for IWS1 antibody 16943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ChIP

Cell Host Microbe

The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells.

Authors - Annabelle Gérard
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sarah (Verified Customer) (12-31-2020)

Works perfectly in WB and immunoprecipitation experiements (HeLa and HEL293T cells).

  • Applications: Western Blot, Immunoprecipitation, ChIP
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEK293T