Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, NIH/3T3 cells |
| Positive IHC detected in | human skin cancer tissue, human thymus tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 4 publications below |
| IF | See 7 publications below |
Product Information
55328-1-AP targets Involucrin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | involucrin |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 80-90 kDa, 56-70 kDa |
| GenBank accession number | NM_005547 |
| Gene Symbol | Involucrin |
| Gene ID (NCBI) | 3713 |
| RRID | AB_11182270 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07476 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)
Publications
| Species | Application | Title |
|---|---|---|
J Eur Acad Dermatol Venereol Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi. | ||
Cell Mol Gastroenterol Hepatol Unraveling the Oncogenic Characteristics of the Cytolinker, Plectin, in Esophageal Squamous Cell Carcinoma | ||
J Ethnopharmacol The Water Extracts from the Oil Cakes of Prinsepia utilis Repair the Epidermal Barrier via Up-Regulating Corneocyte Envelope-Proteins, Lipid Synthases, and Tight Junction Proteins | ||
Life Sci The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model. | ||
Mar Drugs Fucoidan from Undaria pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation. | ||
Biomolecules Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |