Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10822-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 10822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10822-1-AP
IP result of anti-IVD (IP:10822-1-AP, 4ug; Detection:10822-1-AP 1:300) with MCF-7 cells lysate 800ug.
IHC staining of human liver cancer using 10822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10822-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IVD antibody (10822-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

IVD Polyclonal antibody

IVD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10822-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, human brain tissue, mouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below

Product Information

10822-1-AP targets IVD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat, arabidopsis
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1252 Product name: Recombinant human IVD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-423 aa of BC017202 Sequence: MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH 相同性解析による交差性が予測される生物種
Full Name	isovaleryl Coenzyme A dehydrogenase
Calculated molecular weight	46 kDa
Observed molecular weight	41-46 kDa
GenBank accession number	BC017202
Gene Symbol	IVD
Gene ID (NCBI)	3712
RRID	AB_2296258
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26440
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for IVD antibody 10822-1-AP	Download protocol
IHC protocol for IVD antibody 10822-1-AP	Download protocol
IP protocol for IVD antibody 10822-1-AP	Download protocol
WB protocol for IVD antibody 10822-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Arabidopsis	WB	Plant J PMID: 19929878 Authors - Gu Liping L View Article
mouse	WB	Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. Authors - Min-Zhi Peng View Article
mouse	WB	Mol Endocrinol Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo. Authors - Signe Horn View Article
mouse	WB	J Neurochem Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure. Authors - Ryu Myung-Jeom MJ View Article
	WB	Plant Cell Rep The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures. Authors - Danielle S Brito View Article
rat	WB	Proteomics Clin Appl Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Authors - Kelly L Stauch View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10822-1-AP

WB analysis using 10822-1-AP

WB analysis of human brain using 10822-1-AP

WB analysis using 10822-1-AP

IP experiment of MCF-7 using 10822-1-AP

IHC staining of human liver cancer using 10822-1-AP

IHC staining of human liver cancer using 10822-1-AP

IHC staining of human liver cancer using 10822-1-AP

IHC staining of human liver cancer using 10822-1-AP

IF Staining of MCF-7 using 10822-1-AP

IVD Polyclonal antibody

IVD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 19929878

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo.

Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure.

The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures.

Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.