CoraLite® Plus 488-conjugated ITK Monoclonal antibody
ITK Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F9D8
Cat no : CL488-67703
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67703 targets ITK in IF applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ITK fusion protein Ag17087 相同性解析による交差性が予測される生物種 |
| Full Name | IL2-inducible T-cell kinase |
| Calculated molecular weight | 620 aa, 72 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC109077 |
| Gene symbol | ITK |
| Gene ID (NCBI) | 3702 |
| RRID | AB_2919525 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ITK antibody CL488-67703 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
