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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ITGA6X1A Polyclonal antibody (20231-1-AP)

    WB analysis of mouse spleen using 20231-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20231-1-AP (ITGA6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using ITGA6X1A Polyclonal antibody (20231-1-AP)

    WB analysis of mouse colon using 20231-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20231-1-AP (ITGA6-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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ITGA6X1A Polyclonal antibody

ITGA6X1A Polyclonal Antibody for WB, ELISA
Cat No. 20231-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CD49f, Integrin alpha 6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse spleen tissue, mouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20231-1-AP targets ITGA6X1A in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 6
Calculated molecular weight 127 kDa
Observed molecular weight 100 kDa, 150 kDa
GenBank accession numberNM_000210
Gene Symbol Integrin alpha 6
Gene ID (NCBI) 3655
RRIDAB_10665420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGA6, also named as CD49f and VLA-6, belongs to the integrin alpha chain family. It is a receptor for laminin on platelets. ITGA6 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Defects in ITGA6 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) which also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. The antibody is specific to isoform Alpha-6X1A, isoform Alpha-6X2A, isoform Alpha-6X1X2A isoform7 and isoform9 of ITGA6.

Protocols

Product Specific Protocols
WB protocol for ITGA6X1A antibody 20231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols