Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 10569-1-AP
WB result of Integrin alpha-5 antibody (10569-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.
WB analysis using 10569-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10569-1-AP (Integrin alpha-5/CD49e antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10569-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 10569-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 10569-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10569-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10569-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 10569-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10569-1-AP (Integrin alpha-5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 10569-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10569-1-AP (Integrin alpha-5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 10569-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

Integrin alpha-5/CD49e Polyclonal antibody

Integrin alpha-5/CD49e Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10569-1-AP

Publications(67)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (67)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue, Calu-1 cells, NIH/3T3 cells
Positive IHC detected in	human placenta tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 43 publications below
IHC	See 6 publications below
IF	See 14 publications below

Product Information

10569-1-AP targets Integrin alpha-5/CD49e in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity	human, mouse, monkey
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0860 Product name: Recombinant human Integrin alpha-5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 749-1049 aa of BC008786 Sequence: FTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated molecular weight	115 kDa
Observed molecular weight	135-150 kDa
GenBank accession number	BC008786
Gene Symbol	Integrin alpha 5
Gene ID (NCBI)	3678
RRID	AB_2130060
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08648
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Protocols

Product Specific Protocols
IHC protocol for Integrin alpha-5/CD49e antibody 10569-1-AP	Download protocol
WB protocol for Integrin alpha-5/CD49e antibody 10569-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Adv Mater Tracing Immunological Interaction in Trimethylamine N-Oxide Hydrogel-Derived Zwitterionic Microenvironment During Promoted Diabetic Wound Regeneration Authors - Zheng Li View Article
mouse,human	IHC	Nat Commun The CCL20-integrin α5β1 interaction enhances TGF-β/Smad signaling to promote fibroblast activation in pulmonary fibrosis. Authors - Suosi Liu View Article
mouse	WB,IF	Nat Commun In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment Authors - Wangxiao He View Article
rat	IF	J Nanobiotechnology The bone marrow mesenchymal stem cells derived migrasomes induced by Titania nanotubes surface serve as chemotaxis effect for osteogenesis. Authors - Guangwen Li View Article
rat	IHC	J Nanobiotechnology Mechanically tunable fiber-based hydrogel activates PIEZO1-integrin axis for enhanced bone repair. Authors - Jinghong Yang View Article
human	WB	Adv Sci (Weinh) Injectable pH Responsive Conductive Hydrogel for Intelligent Delivery of Metformin and Exosomes to Enhance Cardiac Repair after Myocardial Ischemia-Reperfusion Injury Authors - Nianlan Cheng View Article

Validation Data Gallery

WB analysis of HeLa using 10569-1-AP

WB analysis using 10569-1-AP

WB analysis of human placenta using 10569-1-AP

WB analysis of COS-7 using 10569-1-AP

WB analysis of 3T3-L1 using 10569-1-AP

WB analysis of HeLa using 10569-1-AP

WB analysis of A549 using 10569-1-AP

IHC staining of human placenta using 10569-1-AP

IHC staining of human placenta using 10569-1-AP

IHC staining of human skin cancer using 10569-1-AP

Integrin alpha-5/CD49e Polyclonal antibody

Integrin alpha-5/CD49e Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tracing Immunological Interaction in Trimethylamine N-Oxide Hydrogel-Derived Zwitterionic Microenvironment During Promoted Diabetic Wound Regeneration

The CCL20-integrin α5β1 interaction enhances TGF-β/Smad signaling to promote fibroblast activation in pulmonary fibrosis.

In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment

The bone marrow mesenchymal stem cells derived migrasomes induced by Titania nanotubes surface serve as chemotaxis effect for osteogenesis.

Mechanically tunable fiber-based hydrogel activates PIEZO1-integrin axis for enhanced bone repair.

Injectable pH Responsive Conductive Hydrogel for Intelligent Delivery of Metformin and Exosomes to Enhance Cardiac Repair after Myocardial Ischemia-Reperfusion Injury

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