Validation Data Gallery

Filter: AllWBIHC

WB analysis using 22146-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22146-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22146-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 22146-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22146-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 22146-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 22146-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 22146-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 22146-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22146-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, SMMC-7721 cells, SW480 cells, HT-29 cells
Positive IHC detected in	human liver tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 11 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

22146-1-AP targets Integrin alpha 1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17584 Product name: Recombinant human Integrin alpha-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 792-1141 aa of BC137121 Sequence: DSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 1
Calculated molecular weight	1179 aa, 131 kDa
Observed molecular weight	180 kDa
GenBank accession number	BC137121
Gene Symbol	Integrin alpha 1
Gene ID (NCBI)	3672
RRID	AB_2879008
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P56199
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 1 antibody 22146-1-AP	Download protocol
IHC protocol for Integrin alpha 1 antibody 22146-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Mediators Inflamm ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma Authors - Yanhong Ren View Article
human	WB	Int J Biol Sci Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer. Authors - Hai Li KD Validated View Article
human	WB	Int J Oncol Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules Authors - AMINA BOLATKAN View Article
human	WB	J Ethnopharmacol A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells. Authors - Meng-Meng Wei View Article
human	WB,IHC,IF	Int J Oncol FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins. Authors - Junfen Xu View Article
rat	WB,IF	J Biomech Integrin endocytosis on elastic substrates mediates mechanosensing. Authors - Jing Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22146-1-AP

WB analysis of HepG2 using 22146-1-AP

WB analysis of SMMC-7721 using 22146-1-AP

WB analysis of HeLa using 22146-1-AP

IHC staining of human liver using 22146-1-AP

IHC staining of human liver using 22146-1-AP

IHC staining of human tonsillitis using 22146-1-AP

IHC staining of human tonsillitis using 22146-1-AP

Integrin alpha 1 Polyclonal antibody

Integrin alpha 1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma

Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules

A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Integrin endocytosis on elastic substrates mediates mechanosensing.