Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, HepG2 cells, rat testis tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
14142-1-AP targets ISYNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5272 Product name: Recombinant human ISYNA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-558 aa of BC066902 Sequence: RDIRDFRSSAGLDKVIVLWTANTERFCEVIPGLNDTAENLLRTIELGLEVSPSTLFAVASILEGCAFLNGSPQNTLVPGALELAWQHRVFVGGDDFKSGQTKVKSVLVDFLIGSGLKTMSIVSYNHLGNNDGENLSAPLQFRSKEVSKSNVVDDMVQSNQVLYTPGEEPDHCVVIKYVPYVGDGKRALDEYTSELMLGGTNTLVLHNTCEDSLLAAPIMLDLALLTELCQRVSFCTDMDPEPQTFHPVLSLLSFLFKAPLVPRGSPVVNALFRQRSCIENILRACVGLPPQNHMLLEHKMERPGPSLKRVGPVAATYPMLNKKGPVPAATNGCTGDANGHLQEEPPMPTT 相同性解析による交差性が予測される生物種 |
| Full Name | inositol-3-phosphate synthase 1 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC066902 |
| Gene Symbol | ISYNA1 |
| Gene ID (NCBI) | 51477 |
| RRID | AB_2233801 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ISYNA1 antibody 14142-1-AP | Download protocol |
| IHC protocol for ISYNA1 antibody 14142-1-AP | Download protocol |
| WB protocol for ISYNA1 antibody 14142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway. | ||
Front Pharmacol Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments | ||
Alcohol Clin Exp Res Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma. | ||
Mol Reprod Dev Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice. | ||
Biomed Pharmacother Sulforaphane decreases oxidative stress and inhibits NLRP3 inflammasome activation in a mouse model of ulcerative colitis |
