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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRAK3 Polyclonal antibody

IRAK3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28766-1-AP

Synonyms

ASRT5, FLJ13601, IRAK 3, IRAK M, IRAK3, IRAKM

Validation Data Gallery

Applications:
  • WB analysis using 28766-1-AP

    WB analysis using 28766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 28766-1-AP

    IHC staining of mouse colon using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included
  • IHC staining of rat small intestine using 28766-1-AP

    IHC staining of rat small intestine using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRAK3 antibody included

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRAK3 Ready-To-Use IHC Kit. IRAK3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

28766-1-AP targets IRAK3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK3 fusion protein Ag30309 相同性解析による交差性が予測される生物種
Full Name interleukin-1 receptor-associated kinase 3
Calculated molecular weight 68 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC057800
Gene symbol IRAK3
Gene ID (NCBI) 11213
RRIDAB_2881211
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IRAK3 antibody 28766-1-APDownload protocol
IHC protocol for IRAK3 antibody 28766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols