Validation Data Gallery

Filter: AllWBIHCIF

WB analysis of MCF-7 using 10585-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10585-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10585-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10585-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10585-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10585-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10585-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10585-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10585-1-AP (INVS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10585-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human breast cancer using 10585-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 10585-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10585-1-AP (INVS antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human hepatocirrhosis using 10585-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hepatocirrhosis using 10585-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 10585-1-AP (INVS antibody) at dilution of 1:50 (under 40x lens).
IF Staining of hTERT-RPE1 cells using 10585-1-AP
IF result from Dr. Corbit, Kevin. anti-INVS (also named as NPHP2; 10585-1-AP) marks the ‘inversin compartment’ and basal bodies of Human hTERT-RPE1 cells.
IF Staining of HeLa using 10585-1-AP
Immunofluorescent analysis of Hela cells, using INVS antibody 10585-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

INVS Polyclonal antibody

INVS Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 10585-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

INV, inversin, INVS, KIAA0573, Nephrocystin 2

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (12)

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, K-562 cells, L02 cells, SH-SY5Y cells, mouse skeletal muscle tissue, HepG2 cells, Raji cells, Jurkat cells
Positive IHC detected in	human breast cancer tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, hTERT-RPE1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 5 publications below
FC	See 1 publications below

Product Information

10585-1-AP targets INVS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0934 Product name: Recombinant human INVS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-101 aa of BC006370 Sequence: MNKSENLLFAGSSLASQVHAAAVNGDKGALQRLIVGNSALKDKEDQFGRTPLMYCVLADRLDCADALLKAGADVNKTDHSQRTALHLAAQKALRTISTGRI 相同性解析による交差性が予測される生物種
Full Name	inversin
Calculated molecular weight	118 kDa, 100 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC006370
Gene Symbol	INVS
Gene ID (NCBI)	27130
RRID	AB_2233902
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y283
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.

Publications

Species	Application	Title
rat	WB	Cell Death Dis Planar cell polarity protein Dishevelled 3 (Dvl3) regulates ectoplasmic specialization (ES) dynamics in the testis through changes in cytoskeletal organization. Authors - Linxi Li View Article
		Methods Cell Biol Single-molecule imaging in the primary cilium Authors - Lucien E Weiss View Article
	IF	Proc Natl Acad Sci U S A A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia. Authors - Natalie Acker View Article
mouse	WB,IF	EMBO J Cell type-specific regulation of ciliary transition zone assembly in vertebrates. Authors - Antonia Wiegering KO Validated View Article
human	FC	JCI Insight Cilia proteins are biomarkers of altered flow in the vasculature. Authors - Ankan Gupta View Article
	WB,IF	Ecotoxicol Environ Saf Peptide CyRL-QN15 alleviates UVB-induced skin aging by promoting the repair of barrier Authors - Zijian Kang View Article

Validation Data Gallery

WB analysis of MCF-7 using 10585-1-AP

WB analysis of mouse skeletal muscle using 10585-1-AP

WB analysis of K-562 using 10585-1-AP

WB analysis of HepG2 using 10585-1-AP

WB analysis of Raji using 10585-1-AP

WB analysis of Jurkat using 10585-1-AP

WB analysis of L02 using 10585-1-AP

WB analysis of SH-SY5Y using 10585-1-AP

IHC staining of human breast cancer using 10585-1-AP

IHC staining of human breast cancer using 10585-1-AP

IHC staining of human breast cancer using 10585-1-AP

IHC staining of human hepatocirrhosis using 10585-1-AP

IHC staining of human hepatocirrhosis using 10585-1-AP

IF Staining of hTERT-RPE1 cells using 10585-1-AP

IF Staining of HeLa using 10585-1-AP

INVS Polyclonal antibody

INVS Polyclonal Antibody for WB, IF, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

Planar cell polarity protein Dishevelled 3 (Dvl3) regulates ectoplasmic specialization (ES) dynamics in the testis through changes in cytoskeletal organization.

Single-molecule imaging in the primary cilium

A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia.

Cell type-specific regulation of ciliary transition zone assembly in vertebrates.

Cilia proteins are biomarkers of altered flow in the vasculature.

Peptide CyRL-QN15 alleviates UVB-induced skin aging by promoting the repair of barrier

Able™

Able^™