Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
18802-1-AP targets INTS8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | integrator complex subunit 8 |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 95-111 kDa |
| GenBank accession number | BC136754 |
| Gene Symbol | INTS8 |
| Gene ID (NCBI) | 55656 |
| RRID | AB_10597250 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q75QN2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrator complex subunit 8 (INTS8) is one of the major components of RNA polymerase II and has been demonstrated to be involved in the cleavage of small nuclear RNAs and transcriptional processes (PMID: 34880328). INTS8 was robustly increased in neurodevelopmental diseases and numerous tumors. High INTS8 expression has a tight correlation with poor prognosis in multi-cancers (PMID: 30881114). Western blot analysis detected INTS8 at an apparent molecular mass of 111 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for INTS8 antibody 18802-1-AP | Download protocol |
| IP protocol for INTS8 antibody 18802-1-AP | Download protocol |
| WB protocol for INTS8 antibody 18802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. | ||
J Exp Clin Cancer Res Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma. | ||
Cancer Manag Res INTS8 accelerates the epithelial-to-mesenchymal transition in hepatocellular carcinoma by upregulating the TGF-β signaling pathway. | ||
Cell Rep A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder | ||
Elife Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9 |
