Validation Data Gallery

Filter: AllWBIHC

WB analysis of A549 using 22115-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22115-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 22115-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22115-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 22115-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22115-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells
Positive IHC detected in	human liver tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

22115-1-AP targets INSIG1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17420 Product name: Recombinant human INSIG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-90 aa of BC001880 Sequence: MPRLHDHFWSCSCAHSARRRGPPRASAAGLAAKVGEMINVSVSGPSLLAAHGAPDADPAPRGRSAAMSGPEPGSPYPNTWHHRLLQRSLV 相同性解析による交差性が予測される生物種
Full Name	insulin induced gene 1
Calculated molecular weight	277 aa, 30 kDa
Observed molecular weight	28-31 kDa, 66-70 kDa
GenBank accession number	BC001880
Gene Symbol	INSIG1
Gene ID (NCBI)	3638
RRID	AB_11183051
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15503
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Protocols

Product Specific Protocols
IHC protocol for INSIG1 antibody 22115-1-AP	Download protocol
WB protocol for INSIG1 antibody 22115-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. Authors - Daqian Xu KD Validated View Article
	WB	Transl Psychiatry A potential mechanism underlying atypical antipsychotics-induced lipid disturbances. Authors - H L Cai View Article
	WB	Eur Neuropsychopharmacol Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway. Authors - Ruili Dang View Article
	WB	Mol Nutr Food Res (-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†). Authors - Hui Cheng View Article
	WB,IHC	J Physiol Biochem Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention. Authors - Shasha Liu View Article
human	IF	JCI Insight Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression. Authors - Yingying Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 22115-1-AP

WB analysis of MCF-7 using 22115-1-AP

WB analysis of A549 using 22115-1-AP

WB analysis of HepG2 using 22115-1-AP

WB analysis of SGC-7901 using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.

(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).

Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.

Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.