Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 10665-1-AP
WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells.
WB analysis of Jurkat using 10665-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10665-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10665-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10665-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10665-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10665-1-AP
IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug.
IHC staining of human colon cancer using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human thyroid cancer using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat skin using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10665-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

ING5 Polyclonal antibody

ING5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10665-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Inhibitor of growth protein 5, p28ING5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 22 publications below
IHC	See 10 publications below
IF	See 5 publications below

Product Information

10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1059 Product name: Recombinant human ING5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC005370 Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK 相同性解析による交差性が予測される生物種
Full Name	inhibitor of growth family, member 5
Calculated molecular weight	28 kDa
Observed molecular weight	28-32 kDa
GenBank accession number	BC005370
Gene Symbol	ING5
Gene ID (NCBI)	84289
RRID	AB_2280522
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WYH8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

Protocols

Product Specific Protocols
IHC protocol for ING5 antibody 10665-1-AP	Download protocol
IP protocol for ING5 antibody 10665-1-AP	Download protocol
WB protocol for ING5 antibody 10665-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Cell Res LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration. Authors - Yahan Wu KD Validated View Article
mouse	WB	EMBO Rep SCFJFK is functionally linked to obesity and metabolic syndrome. Authors - Lin He View Article
human	WB	Oncotarget The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer. Authors - Yang Li View Article
human	WB	Oncotarget ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer. Authors - Feng Zhang View Article
human	IF	Oncotarget ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression. Authors - Wen-feng Gou KD Validated View Article
human	WB,IHC	Oncotarget Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5. Authors - Yiyi Cao KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 10665-1-AP

WB analysis of Jurkat using 10665-1-AP

WB analysis of PC-3 using 10665-1-AP

WB analysis of Jurkat using 10665-1-AP

WB analysis of HEK-293 using 10665-1-AP

WB analysis of mouse kidney using 10665-1-AP

IP experiment of HEK-293 using 10665-1-AP

IHC staining of human colon cancer using 10665-1-AP

IHC staining of human thyroid cancer using 10665-1-AP

IHC staining of mouse kidney using 10665-1-AP

IHC staining of rat skin using 10665-1-AP

IHC staining of human ovary tumor using 10665-1-AP

IHC staining of human ovary tumor using 10665-1-AP

ING5 Polyclonal antibody

ING5 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.

SCFJFK is functionally linked to obesity and metabolic syndrome.

The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer.

ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer.

ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression.

Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5.