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INF2 Polyclonal antibody
INF2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 20466-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, NIH/3T3 cells, A431 cells, K-562 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 19 publications below |
| IHC | See 3 publications below |
| IF | See 17 publications below |
| CoIP | See 1 publications below |
Product Information
20466-1-AP targets INF2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INF2 fusion protein Ag14302 相同性解析による交差性が予測される生物種 |
| Full Name | inverted formin, FH2 and WH2 domain containing |
| Calculated molecular weight | 1249 aa, 136 kDa |
| Observed molecular weight | 180-200 kDa |
| GenBank accession number | BC008756 |
| Gene symbol | INF2 |
| Gene ID (NCBI) | 64423 |
| RRID | AB_10694821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INF2 antibody 20466-1-AP | Download protocol |
| IHC protocol for INF2 antibody 20466-1-AP | Download protocol |
| IF protocol for INF2 antibody 20466-1-AP | Download protocol |
| IP protocol for INF2 antibody 20466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis. | ||
Nat Commun GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics. | ||
Proc Natl Acad Sci U S A Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly. | ||
Proc Natl Acad Sci U S A Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sammy (Verified Customer) (11-05-2023) | Excellent antibody. Detects a band at the correct MW.
 |
FH Chun (Verified Customer) (07-03-2019) | This antibody is good.
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