Validation Data Gallery
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Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 4 publications below |
Product Information
26677-1-AP targets IL-5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24583 Product name: Recombinant human IL-5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-101 aa of BC066282 Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQ 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 5 (colony-stimulating factor, eosinophil) |
| Calculated molecular weight | 134 aa, 15 kDa |
| GenBank accession number | BC066282 |
| Gene Symbol | IL5 |
| Gene ID (NCBI) | 3567 |
| RRID | AB_2880598 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05113 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-5 antibody 26677-1-AP | Download protocol |
| WB protocol for IL-5 antibody 26677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Laryngoscope Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy. | ||
J Immunol Res Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p. | ||
J Crohns Colitis Mucosal cytokine expression associated with deep endoscopic mucosal healing in ulcerative colitis | ||
Otolaryngol Head Neck Surg Elevated Body Mass Index Aggravates Histopathological Changes and Postoperative Recurrence Risk in Nasal Polyps |
