IL-4R Polyclonal antibody

IL-4R Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 28331-1-AP

Synonyms

CD124, IL 4 binding protein, IL 4 receptor subunit alpha, IL 4R alpha, IL 4R subunit alpha, IL 4RA, IL4 BP, IL4R, IL-4R, IL4RA, interleukin 4 receptor, sIL4Ralpha/prot, Soluble IL 4R alpha



Tested Applications

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28331-1-AP targets IL-4R in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-4R fusion protein Ag28766 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight 90 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberNM_000418
Gene symbol IL4R
Gene ID (NCBI) 3566
RRIDAB_2881115
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-4R antibody 28331-1-APDownload protocol
IHC protocol for IL-4R antibody 28331-1-APDownload protocol
FC protocol for IL-4R antibody 28331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomedicines

Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis

Authors - Ying Jiang