IL-1 Alpha Polyclonal antibody

IL-1 Alpha Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16765-1-AP

Synonyms

Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL-1, IL1 ALPHA, IL-1 alpha, IL1A, IL1F1, IL-1α, Interleukin 1 alpha, interleukin 1, alpha



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells, human placenta tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy IL1A Ready-To-Use IHC Kit. IL1A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16765-1-AP targets IL-1 Alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 Alpha fusion protein Ag10339 相同性解析による交差性が予測される生物種
Full Name interleukin 1, alpha
Calculated molecular weight 271 aa, 31 kDa
Observed molecular weight30 kDa
GenBank accession numberBC013142
Gene symbol IL1A
Gene ID (NCBI) 3552
RRIDAB_10641044
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Protocols

Product Specific Protocols
WB protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
IHC protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
IF protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
humanIF

Nat Commun

Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment

Authors - Meilin Xue
humanWB

Gut Microbes

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Authors - Jian-Wei Zhang

Cancer Lett

Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer

Authors - Prabhakar Pitta Venkata
ratWB

Bioact Mater

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Authors - Liwen Luo
humanIF

Cell Death Discov

Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.

Authors - Lulin Zhou