IL-17A Polyclonal antibody

IL-17A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 13082-1-AP

Synonyms

CTLA 8, CTLA8, IL 17, IL 17A, IL17, IL-17, IL17A, interleukin 17A



Tested Applications

Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human stomach cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13082-1-AP targets IL-17A in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-17A fusion protein Ag3733 相同性解析による交差性が予測される生物種
Full Name interleukin 17A
Calculated molecular weight 155 aa, 18 kDa
GenBank accession numberBC067505
Gene symbol IL17A
Gene ID (NCBI) 3605
RRIDAB_10644322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased. This antibody got 32-35 kDa band in western blot, maybe due to homodimer formation and differential glycosylations.

Protocols

Product Specific Protocols
IHC protocol for IL-17A antibody 13082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Autoimmun

IL-17 is expressed on beta and alpha cells of donors with type 1 and type 2 diabetes.

Authors - Sakthi Rajendran
WB

J Med Chem

Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease.

Authors - Ji-An Chen
IHC

J Invest Dermatol

Reduced Il17a expression distinguishes a Ly6c(lo)MHCII(hi) macrophage population promoting wound healing.

Authors - Rodero Mathieu P MP
IF

Arch Oral Biol

Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium.

Authors - Koichiro Irie
WB

Cell Physiol Biochem

Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.

Authors - Yang Yang
WB

J Cell Mol Med

Human breast milk-derived exosomes through inhibiting AT II cell apoptosis to prevent bronchopulmonary dysplasia in rat lung.

Authors - Yahui Zhou