Validation Data Gallery
Tested Applications
| Positive IHC detected in | human oesophagus tissue,  human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 5 publications below | 
Product Information
21255-1-AP targets IL-36 Gamma/IL-1F9 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Cited Reactivity | human | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag15804 Product name: Recombinant human IL-1F9/IL-36 gamma protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-95 aa of BC098337 Sequence: MRGTPGDADGGGRAVYQSMCKPITGTINDLNQQVWTLQGQNLVAVPRSDSVTPVTVAVITCKYPEALEQGRGDPIYLGIQNPEMCLYCEKVGEQP相同性解析による交差性が予測される生物種 | 
| Full Name | interleukin 1 family, member 9 | 
| Calculated molecular weight | 169 aa, 19 kDa | 
| GenBank accession number | BC098337 | 
| Gene Symbol | IL-36 gamma/IL-1F9 | 
| Gene ID (NCBI) | 56300 | 
| RRID | AB_2878832 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q9NZH8 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-36 Gamma/IL-1F9 antibody 21255-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Clin Exp Immunol Distinct Expression of IL-36α, β, γ, their antagonist IL-36Ra and IL-38 in psoriasis, rheumatoid arthritis and Crohn's disease. | ||
| Exp Dermatol Clinical Responses and Transcriptomic Analysis of Spesolimab in a Girl With Severe Dermatitis, Multiple Allergies and Metabolic Wasting Syndrome | 




