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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC, ELISA

Conjugate

Unconjugated

Cat no : 30199-1-AP

Synonyms

Il2, IL-2, interleukin 2

Validation Data Gallery

Applications:
  • FC experiment of rat splenocytes using 30199-1-AP

    FC experiment of rat splenocytes using 30199-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of rat splenocytes using 30199-1-AP

    FC experiment of rat splenocytes using 30199-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8), and 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of rat splenocytes using 30199-1-AP

    FC experiment of rat splenocytes using 30199-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8), and 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPMA and ionomycin treated rat splenocytes cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

30199-1-AP targets IL-2 in FC, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight18kd
GenBank accession numberNM_053836
Gene symbol Il2
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
FC protocol for IL-2 antibody 30199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols