• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B2F5

Cat no : CL488-60319

Synonyms

FIL1 theta, FIL1T, FKSG75, IL 1 theta, IL 1F10, IL 1HY2, IL1 theta, IL1F10, IL1HY2, Interleukin 1 family member 10, Interleukin 1 HY2, Interleukin 1 theta, UNQ6119/PRO20041

Validation Data Gallery

Applications:
  • FC experiment of U-937 using CL488-60319

    FC experiment of U-937 using CL488-60319

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 60319-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60319 targets IL-1F10 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1F10 fusion protein Ag17250 相同性解析による交差性が予測される生物種
Full Name interleukin 1 family, member 10 (theta)
Calculated molecular weight 152 aa, 17 kDa
GenBank accession numberBC103966
Gene symbol IL1F10
Gene ID (NCBI) 84639
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IL-1F10 antibody CL488-60319Download protocol
Standard Protocols
Click here to view our Standard Protocols