Human IgG4 Monoclonal antibody
Human IgG4 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A1A2
Cat no : 66408-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | human plasma tissue, human heart tissue, human skeletal muscle tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human appendicitis tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
Product Information
66408-1-Ig targets Human IgG4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant gamma 4 (G4m marker) |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | AL928742 |
| Gene symbol | IGHG4 |
| Gene ID (NCBI) | 3503 |
| RRID | AB_2881780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgG4 antibody 66408-1-Ig | Download protocol |
| IHC protocol for Human IgG4 antibody 66408-1-Ig | Download protocol |
| IF protocol for Human IgG4 antibody 66408-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Breast Cancer Res Clinical and biological heterogeneities in triple-negative breast cancer reveals a non-negligible role of HER2-low |
