Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse colon tissue, human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 24 publications below |
| IHC | See 5 publications below |
| IF | See 10 publications below |
Product Information
20253-1-AP targets IGF2R-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | insulin-like growth factor 2 receptor |
| Calculated molecular weight | 274 kDa |
| Observed molecular weight | 300 kDa |
| GenBank accession number | NM_000876 |
| Gene Symbol | IGF2R |
| Gene ID (NCBI) | 3482 |
| RRID | AB_10859779 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11717 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IF protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IHC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| IP protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| WB protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Salvianolic Acid B Upregulates miR-744-5p Expression in Peripheral Blood Mononuclear Cell-Derived Extracellular Vesicles to Alleviate Uremic Cardiomyopathy. | ||
Nat Commun GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway | ||
J Adv Res CD34+CLDN5+ tumor associated senescent endothelial cells through IGF2-IGF2R signaling increased cholangiocellular phenotype in hepatocellular carcinoma | ||
Metabolism Nonenzymatic function of DPP4 promotes diabetes-associated cognitive dysfunction through IGF-2R/PKA/SP1/ERp29/IP3R2 pathway-mediated impairment of Treg function and M1 microglia polarization | ||
Redox Biol Sulfhydrated TFEB alleviates blast-induced lung injury by maintaining epithelial barrier integrity through the IGF2R/MMP-2/9 pathway. |