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IFT88 抗体 265 文献

Rabbit Polyclonal| カタログ番号: 13967-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, CoIP
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Images See all

IFT88 Antibody WB HEK-293 cells 13967-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IFT88 Antibody IF MDCK cells 13967-1-AP

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13967-1-AP (IFT88 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IFT88 Antibody IP knockout cells and WT cells 13967-1-AP

IP and WB result of IFT88 (13967-1-AP) from Dr. Corbit, Kevin. Knockout cells and WT cells.

IFT88 Antibody IF hTERT-RPE1 cells 13967-1-AP

IF result of anti-IFT88 (13967-1-AP) from Dr. Corbit, Kevin and Dr. Andrew Kodani.

IFT88 Antibody IHC human heart tissue 13967-1-AP

Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 10x lens).

IFT88 Antibody IHC human heart tissue 13967-1-AP

Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 40x lens).

種特異性:
human, mouse, rat, Canine, chicken, pig, zebrafish
WB ポジティブ:
HEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells
IP ポジティブ:
knockout cells and WT cells, HEK-293 cells
IHC ポジティブ:
human heart tissue, human pancreas tissue
IF ポジティブ:
MDCK cells, hTERT-RPE1 cells
推奨希釈倍率:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
IFT88 fusion protein Ag4980
正式名:
intraflagellar transport 88 homolog (Chlamydomonas)
計算分子量:
94 kDa
検出分子量:
88-95 kDa
GenBank accession number:
BC030776
Gene ID (NCBI):
8100
遺伝子シンボル:
IFT88
別名:
IFT88, TPR repeat protein 10, TTC10

背景説明

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.


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