PE Anti-Mouse IFN gamma (XMG1.2)

IFN gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse and More (1)

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

XMG1.2

Cat no : PE-65153

Synonyms

Ifg, IFN g, IFN gamma, Ifng, IFN-gamma, IFNγ, IFN-γ, interferon gamma



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65153 targets IFN gamma in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivity mouse
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen E. coli-expressed, recombinant mouse IFN-γ 相同性解析による交差性が予測される生物種
Full Name interferon gamma
Calculated molecular weight17 kDa
GenBank accession numberBC119063
Gene symbol Ifng
Gene ID (NCBI) 15978
RRIDAB_2883932
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protocols

Product Specific Protocols
FC protocol for PE IFN gamma antibody PE-65153Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC (Intra)

Phytomedicine

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Authors - Li Wu
FC (Intra)

Nat Commun

CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis

Authors - Ping Yang
mouseFC (Intra)

ACS Nano

Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity

Authors - Yunkai Shang
mouseFC (Intra)

Cell Mol Gastroenterol Hepatol

Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation

Authors - Shan Fu
mouseFC (Intra)

J Med Chem

Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer

Authors - Linxiang Cai