FITC Plus Anti-Mouse IFN gamma (XMG1.2)
IFN gamma Monoclonal Antibody for FC (Intra)
Host / Isotype
Rat / IgG1, kappa
Reactivity
Mouse and More (1)
Applications
FC (Intra)
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
XMG1.2
Cat no : FITC-65153
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | PMA and ionomycin stimulated Th1-polarized splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Product Information
FITC-65153 targets IFN gamma in FC (Intra) applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Purified Recombinant Mouse IFN gamma 相同性解析による交差性が予測される生物種 |
| Full Name | interferon gamma |
| Calculated molecular weight | 17 kDa |
| GenBank accession number | BC119063 |
| Gene symbol | Ifng |
| Gene ID (NCBI) | 15978 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus IFN gamma antibody FITC-65153 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Biomater Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment |
