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  • KD/KO Validated

IFITM3 Polyclonal antibody

IFITM3 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11714-1-AP

Synonyms

1 8U, Fragilis, IFITM3, IP15



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, THP-1 cells
Positive IP detected inHepG2 cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, goat, chicken, rat, mouse, pig, canine, African green monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM3 fusion protein Ag2285 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight 133 aa, 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006794
Gene symbol IFITM3
Gene ID (NCBI) 10410
RRIDAB_2295684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM3 antibody 11714-1-APDownload protocol
IF protocol for IFITM3 antibody 11714-1-APDownload protocol
IP protocol for IFITM3 antibody 11714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

A human apolipoprotein L with detergent-like activity kills intracellular pathogens.

Authors - Ryan G Gaudet
human,mouseWB

Cell

A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.

Authors - Noémie Alphonse
humanWB

Nature

IFITM3 restricts the morbidity and mortality associated with influenza.

Authors - Everitt AR
mouseIHC

Science

T cell memory. Skin-resident memory CD8⁺ T cells trigger a state of tissue-wide pathogen alert.

Authors - Silvia Ariotti
humanIF

Cell Stem Cell

Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.

Authors - Galina Popova
humanWB,IF

Cell Stem Cell

PMID: 30416070

Authors - Carolina Petrillo
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Thomas (Verified Customer) (06-29-2022)

Works fine in WB and IF

  • Applications: Western Blot, Immunofluorescence
FH

Xiuqing (Verified Customer) (03-21-2022)

worked very well for western blot

  • Applications: Western Blot
  • Cell Tissue Type: MARC-145 cells, H1299 cells