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  • KD/KO Validated

IFITM1 Polyclonal antibody

IFITM1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11727-3-AP

Synonyms

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells
Positive IP detected inROS1728 cells
Positive IHC detected inhuman lymphoma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11727-3-AP targets IFITM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, goat, African green monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM1 fusion protein Ag2320 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 1 (9-27)
Calculated molecular weight 14 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC000897
Gene symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2122083
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for IFITM1 antibody 11727-3-APDownload protocol
IHC protocol for IFITM1 antibody 11727-3-APDownload protocol
IP protocol for IFITM1 antibody 11727-3-APDownload protocol
FC protocol for IFITM1 antibody 11727-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Mitotic progression following DNA damage enables pattern recognition within micronuclei.

Authors - Shane M Harding
humanFC

Nature

Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.

Authors - Edward J Grow
  • KD Validated
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanWB

Mol Ther

Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.

Authors - Sarah Aref
humanWB

Nat Commun

IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses

Authors - M. Clement
humanWB

Cell Rep

IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.

Authors - Jingyou Yu
  • KD Validated