Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of human skeletal muscle using 15909-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis using 15909-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15909-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15909-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15909-1-AP
IP result of anti-IDH3A (IP:15909-1-AP, 4ug; Detection:15909-1-AP 1:1000) with HepG2 cells lysate 2400ug.
IHC staining of human brain using 15909-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 15909-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 15909-1-AP
Immunofluorescent analysis of HepG2 cells using 15909-1-AP (IDH3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 15909-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3A antibody (15909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 15909-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IDH3A (15909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15909-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

NAD(+)-specific ICDH subunit alpha, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, IDH3, EC:1.1.1.41

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, human skeletal muscle tissue, mouse brain tissue, rat brain tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 19 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

15909-1-AP targets IDH3A in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8662 Product name: Recombinant human IDH3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC021967 Sequence: MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD 相同性解析による交差性が予測される生物種
Full Name	isocitrate dehydrogenase 3 (NAD+) alpha
Calculated molecular weight	366 aa, 40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC021967
Gene Symbol	IDH3A
Gene ID (NCBI)	3419
RRID	AB_2123282
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50213
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for IDH3A antibody 15909-1-AP	Download protocol
IHC protocol for IDH3A antibody 15909-1-AP	Download protocol
IP protocol for IDH3A antibody 15909-1-AP	Download protocol
WB protocol for IDH3A antibody 15909-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang View Article
mouse	WB	EMBO J Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues. Authors - Yingjiang Xu View Article
human	WB,CoIP	Acta Pharmacol Sin Celastrol inhibits the DPYSL2-JAK/STAT pathway by targeting mito-IDHs mediated mitochondrial metabolism to exhaust breast cancer Authors - Qiao-Li Shi View Article
human	IHC	J Invest Dermatol Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata. Authors - Zhichao Gu View Article
human	WB	Oncotarget CDC5L drives FAH expression to promote metabolic reprogramming in melanoma. Authors - Zhichao Gu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human skeletal muscle using 15909-1-AP

WB analysis using 15909-1-AP

WB analysis of mouse brain using 15909-1-AP

WB analysis of HepG2 using 15909-1-AP

IP experiment of HepG2 using 15909-1-AP

IHC staining of human brain using 15909-1-AP

IHC staining of human brain using 15909-1-AP

IF Staining of HepG2 using 15909-1-AP

IF Staining of HepG2 using 15909-1-AP

FC experiment of HepG2 using 15909-1-AP

IDH3A Polyclonal antibody

IDH3A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A glycolytic shift in Schwann cells supports injured axons.

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

Celastrol inhibits the DPYSL2-JAK/STAT pathway by targeting mito-IDHs mediated mitochondrial metabolism to exhaust breast cancer

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.