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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICOSLG Monoclonal antibody

ICOSLG Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G1F8

Cat no : 67003-1-Ig

Synonyms

B7 H2, B7 homolog 2, B7 like protein Gl50, B7 related protein 1, B7H2, B7RP 1, B7RP1, CD275, GL50, ICOS L, ICOS ligand, ICOSL, ICOSLG, KIAA0653, LICOS

Validation Data Gallery

Applications:
  • WB analysis using 67003-1-Ig

    WB analysis using 67003-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Daudi using 67003-1-Ig

    WB analysis of Daudi using 67003-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHL-60 cells, Daudi cells, HUVEC cells, JAR cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67003-1-Ig targets ICOSLG in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ICOSLG fusion protein Ag6799 相同性解析による交差性が予測される生物種
Full Name inducible T-cell co-stimulator ligand
Calculated molecular weight 33 kDa, 34 kDa
Observed molecular weight 60-65 kDa, 28-35 kDa
GenBank accession numberBC064637
Gene symbol ICOSLG
Gene ID (NCBI) 23308
RRIDAB_2882320
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

Protocols

Product Specific Protocols
WB protocol for ICOSLG antibody 67003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols