CoraLite®594-conjugated IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D3F5

Cat no : CL594-66045

Synonyms

FLJ16131, IA 2, IA 2/PTP, IA2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy PTPRN Ready-To-Use IHC Kit. PTPRN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66045-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66045 targets IA-2/PTPRN in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen IA-2/PTPRN fusion protein Ag17909 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, N
Calculated molecular weight 105 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC007713
Gene symbol PTPRN
Gene ID (NCBI) 5798
RRIDAB_2883490
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Protocols

Product Specific Protocols
IF protocol for CL594 IA-2/PTPRN antibody CL594-66045Download protocol
Standard Protocols
Click here to view our Standard Protocols